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Abstract Objective: This study aimed to investigate the effects of systemic administration of P. eurycarpa Yalt. plant extract on alveolar bone loss and oxidative stress biomarkers in gingival tissue in a rat model of experimental periodontitis. Methodology: 32 male Wistar albino rats, weighing 200-250 g, were divided into four groups (n=8): Healthy control (HC), Experimental periodontitis control (EPC), Experimental periodontitis 400 mg/kg (EP400), Experimental periodontitis 800 mg/kg (EP800). Experimental periodontitis was induced using the ligating method. Distilled water was administered to the HC and EPC groups and the plant extract was administered to the EP400 and EP800 groups by oral gavage at doses of 400 mg/kg and 800 mg/kg, respectively. The rats were sacrificed on the 15th day. The values of glutathione peroxidase GSH-Px, malondialdehyde (MDA), superoxide dismustase (SOD), interleukin-1β (IL-1β), interleukin-10 (IL-10), total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) in the gingival tissues were analyzed by ELISA tests. Alveolar bone loss was assessed using micro-CT images of the maxilla. Results: Although the IL-1β, TOS, OSI results of the healthy control group were lower than those of the other groups, the TAS values were higher (p<0.05). No significant difference was found in the biochemical parameters among the EPC, EP400, and EP800 groups (p>0.05). Alveolar bone loss was significantly reduced in the extract groups compared to the EPC group (p<0.001). Conclusion: Within the limitations of this study, it was observed that the systemic P. eurycarpa extract application reduced alveolar bone loss in a rat model of experimental periodontitis. Further studies are needed to elucidate the beneficial effects of P. eurycarpa.
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ABSTRACT Objective: To postoperatively evaluate knee scores, radiological assessment results, deficit correction, patellar height change, bone healing time, and weight bearing time in patients undergoing high tibial osteotomy (HTO) with/without autologous iliac bone grafting. Methods: This retrospective examination of treated controls from a randomized controlled study included 63 knees of 58 patients aged 46-59 years who underwent HTO with locking open wedge osteotomy plates. The patients were divided into two groups: Group A, HTO with autologous iliac bone grafts (n = 31); and Group B, HTO without autologous iliac bone grafts (n = 32). Clinical and radiological data were evaluated prospectively at the preoperative consultation and again at 6, 9, and 12 weeks, 6 months, and 1 year after the surgery (and annually thereafter). Results: There were no significant intergroup differences in the radiological assessment, deficit correction, patellar height change, bone-healing time, and weight-bearing time at any time after surgery. The knee scores changed positively in both groups (p < 0.001). Conclusions: There was no difference in the results of patients undergoing HTO with open wedge osteotomy titanium locking plates with or without autografting, and comorbidities resulting from autografts were eliminated with the use of locking plates. Level of evidence III, Retrospective Study.
RESUMO Objetivo: Avaliar escores de joelho, resultados da avaliação radiológica, correção de déficits, alteração da altura patelar, tempo de consolidação óssea e tempo para apoio de peso no pós-operatório em pacientes submetidos à osteotomia tibial alta (OTA) com ou sem enxerto autólogo de osso ilíaco. Métodos: O exame retrospectivo de controles tratados em estudo randomizado e controlado foi realizado em 63 joelhos de 58 pacientes com idade entre 46 e 59 anos submetidos a OTA com placas bloqueadas de titânio em cunha aberta. Os pacientes foram divididos em dois grupos: Grupo A, OTA com enxerto de osso ilíaco autólogo (n = 31) e Grupo B, OTA sem enxerto autólogo de osso ilíaco (n = 32). Os dados clínicos e radiológicos foram avaliados prospectivamente na consulta pré-operatória e 6, 9 e 12 semanas e 6 meses e 1 ano após a cirurgia (e depois disso, anualmente). Resultados: Não houve diferenças significativas quanto a avaliação radiológica, correção de déficit, mudança de altura da patela, tempo de cicatrização óssea e tempo para apoio de peso entre os dois grupos em nenhum momento após a cirurgia. Os escores de joelho mudaram positivamente em ambos os grupos (p < 0,001). Conclusões: Não houve diferença nos resultados dos pacientes submetidos a OTA com placas bloqueadas de titânio em cunha aberta com e sem autoenxerto, e as comorbidades resultantes dos autoenxertos foram eliminadas com o uso de placas bloqueadas. Nível de Evidência III, Estudo Retrospectivo.
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Abstract Objective In this study, we aimed to histologically and immunologically evaluate the effect of diode laser treatment when applied adjunctive to scaling and root planing (SRP) in an experimental periodontitis model. Materials and methods We used Wistar-Albino rats (n=60) with average weight of 230 g. Experimental periodontitis was induced by ligature at the right and left first mandibular molar teeth in all rats. After 11 days, the ligature was removed and rats were divided into two groups. The control group (n=30) received only SRP treatment, while the laser group (n=30) received a diode laser (GaAlAs, 810 nm, 1 W, 10 J, 20 s) treatment adjunctive to SRP. Ten rats in each group were sacrificed after 7, 15, and 30 days. Histopathological examination was performed in the left mandible of rats. Myeloperoxidase (MPO) was evaluated by western blot in the gingival specimens from the right mandible. Results MPO levels in the laser group were statistically significantly lower compared with the control group (p≤0.05). There was no statistically significance at any time between MPO levels in the control group (p>0.05). MPO levels in the laser group at the 7th day were statistically significantly higher compared to the 15th (p≤0.05) and the 30th day (p≤0.05). Inflammatory cell infiltration decreased over time in both groups and was statistically significantly lower in the laser group than in the control group at all times (p≤0.01). Conclusions Within the limits of this study, we suggest that diode laser application is an adjunctive treatment because it reduced inflammation and MPO when applied in addition to SRP. On the other hand, more studies are needed for the assessment of the effects of diode laser application to periodontal tissues.
Subject(s)
Animals , Periodontitis/pathology , Periodontitis/therapy , Dental Scaling/methods , Peroxidase/analysis , Low-Level Light Therapy/methods , Lasers, Semiconductor/therapeutic use , Periodontitis , Time Factors , Random Allocation , Blotting, Western , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Combined Modality Therapy , Disease Models, Animal , LigationABSTRACT
BACKGROUND: Traumatic bilateral hip dislocation is a rare condition. Anterior shoulder dislocation combined with this condition is even a rare occurrence. A 57-year-old man had bilateral asymmetric hip dislocation with a posterior wall fracture of the left acetabulum associated with right shoulder anterior dislocation caused by falling down from a cherry tree. Traumatic hip dislocation and anterior shoulder dislocation represent a true orthopedic emergency. METHODS: Closed reduction was performed immediately for three of the dislocated joints. Posterior wall fracture of the left acetabulum was treated with open reduction and internal fixation by screws. Because of the severity of associated complications like aseptic necrosis or posttraumatic osteoarthritis, efforts were made to ensure prompt diagnosis, therapy and prognosis. RESULTS: The patient was successfully treated by open reduction and internal fixation of the left acetabulum, and discharged from the hospital. CONCLUSION: Traumatic asymmetric hip dislocation and anterior shoulder dislocation are an orthopedic emergency and early diagnosis with immediate reduction of the dislocation is the key determinants of excellent result of treatment.